CAPTO MMC IMPRES FULL

BsAb and the hole-hole homodimer, which are different by <1 kDa in size, can be differentiated by non-reducing SDS-PAGE. Mixed-mode resin Capto MMC ImpRes can effectively remove the above mentioned impurities under appropriate conditions. Highlights Half antibody, hole-hole homodimer and aggregates are common by-products associated with the production of KiH-based bsAb. This makes MMC ImpRes a powerful tool for bsAb purification. The adsorption is salt tolerant, meaning that binding of proteins can be. A factor affecting the separation might be the ligand density of the anion exchange resin. In this experiment, 17 mM MgCl 2 (Fig 8C) improved separation compared to 20 mM MgCl 2 (Fig 8D).

CAPTO MMC IMPRES FULL

In this work, with a case study we demonstrated that Capto MMC ImpRes mixed-mode chromatography can effectively remove these two by-products as well as antibody aggregates under optimized conditions. Capto MMC combines recent base matrix developments with innovative ligand chemistry. The MgCl 2 concentration needs to be fine-tuned to maximize the removal of empty capsids while retaining the full capsids for elution in the NaCl gradient. size (median particle size of the cumulative volume distribution) Synonyms: Capto MMC ImpRes. Among them, half antibody and homodimer are found with high frequency. Capto MMC ImpRes, 100 ML Cytiva 17371602, 40 m avg. Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger.

Abstract During recombinant production of asymmetric IgG-like bispecific antibodies (bsAbs), various by-products are often observed due to unbalanced chain expression and incorrect chain pairing. Capto MMC is a multimodal salt-tolerant BioProcess resin for capture and intermediate purification of proteins from large feed volumes by packed bed chromatography.

Capto mmc impres